Extended-depth point localisation microscopy using Airy-beam-based point-spread functions

Hi all!

Imaging Concepts Group's turn this week at the Optical Seminar, with Zhuang taking a break from his thesis to tell us about some of his PhD work:

Title: 

Extended-depth point localisation microscopy using Airy-beam-based point-spread functions

Abstract:

The precise localisation of point sources in optical microscopy enables nm-resolution imaging of single-molecules and biological dynamics. Existing approaches for point localisation, however, suffer from the very limited depth range and thus not suitable for imaging thick samples. We utilise the lateral translation property and the diffraction-free propagation of Airy-beam-based point-spread functions to localise point emitters in 3D space with the key advantages of extended depth range, higher optical throughput and potential for imaging higher emitter densities than are possible using other techniques. The proposed techniques are demonstrated for blood-flow imaging in a live zebrafish. 

Thursday 4th of April, 4pm, room 312 Kelvin Building.

As usual, snacks and soft drinks in the common room afterwards :)

Hope to see many of your there,

Cheers,

Chiara.