23/04/2019

Optical angular momentum transfer on total internal reflection

Hi all,

The next seminar will be held this Thursday 25th April, 3.30 pm, Kelvin Building lecture theatre 257, with Atirach Ritboon from the Quantum Theory group.

Abstract:
  1. We study the mechanism of optical angular momentum transfer from light to a dielectric medium on total internal reflection. We employ a quantized approach and, in particular, work with a single-photon pulse. This allows us to evaluate the force and torque per photon and also, crucially, to evaluate forces and torques conditioned on transmission or reflection at an interface. The reflected electric and magnetic fields of an incident paraxial beam carrying orbital and spin angular momentum are obtained using an angular spectrum method. We calculate the expectation value of the single-photon torque exerted on the dielectric, due to total internal reflection of a single-photon pulse, using the dipole-based Lorentz force density. We apply this result to describe the angular momentum transfer from light on passing through an M-shaped Dove prism. 

Hope to see you there, and for coffee and biscuits in the common room afterwards,

Frances

08/04/2019

Adaptive Prospective Optical Gating Allows 4D Imaging and Quantification in the Developing Zebrafish Heart

Next seminar this Thursday 11th April, 4pm, room 222 Kelvin Building, this time with Chas Nelson from the Imaging Concepts Group!


Title:
Adaptive Prospective Optical Gating Allows 4D Imaging and Quantification in the Developing Zebrafish Heart.
Abstract:
Researchers interested in the heart have long used a wide range of imaging techniques to see, understand and quantify changes throughout heart development, repair and, in certain species, regeneration. Two major challenges in imaging the heart are the contrasting problems of high-frequency heart beating and low-frequency morphological changes. I will demonstrate how using our established method of prospective optical gating, in combination with light-sheet microscopy, can allow the synchronised capture of 3D images of the in vivo beating zebrafish heart. However, prospective optical gating alone is limited to snapshots of the heart at chosen target heartbeat phases and only over the scale of tens of minutes.
We have now developed adaptive prospective optical gating technologies that we are using in combination with light-sheet microscopy to enable a range of 3D+time and 3D-timelapse imaging experiments. Our adaptive prospective optical gating technology allows us to carry out 48+ hour, in vivo, 3D-timelapse imaging of the computationally 'frozen' heart across developmental stages, e.g. heart looping, and throughout injury response and repair. Imaging across these timescales is not possible with prospective optical gating alone and phase-locked timelapse imaging is not possible using retrospective optical gating alone: only with our hybrid system are such longitudinal studies possible. Our hybrid prospective-retrospective optical gating system allows researchers to study and understand cardiac development and repair without the use of chemicals or optogenetics to stop or modify the natural heart beating.

Hope to see many of you there,
Cheers,
Chiara.

01/04/2019

Extended-depth point localisation microscopy using Airy-beam-based point-spread functions



Hi all!

Imaging Concepts Group's turn this week at the Optical Seminar, with Zhuang taking a break from his thesis to tell us about some of his PhD work:


Title: 
Extended-depth point localisation microscopy using Airy-beam-based point-spread functions


Abstract:
The precise localisation of point sources in optical microscopy enables nm-resolution imaging of single-molecules and biological dynamics. Existing approaches for point localisation, however, suffer from the very limited depth range and thus not suitable for imaging thick samples. We utilise the lateral translation property and the diffraction-free propagation of Airy-beam-based point-spread functions to localise point emitters in 3D space with the key advantages of extended depth range, higher optical throughput and potential for imaging higher emitter densities than are possible using other techniques. The proposed techniques are demonstrated for blood-flow imaging in a live zebrafish. 


Thursday 4th of April, 4pm, room 312 Kelvin Building.

As usual, snacks and soft drinks in the common room afterwards :)

Hope to see many of your there,
Cheers,
Chiara.