Title:
Adaptive Prospective Optical Gating Allows 4D Imaging and Quantification in the Developing Zebrafish Heart.
Abstract:
Researchers interested in the heart have long used a wide range of imaging techniques to see, understand and quantify changes throughout heart development, repair and, in certain species, regeneration. Two major challenges in imaging the heart are the contrasting problems of high-frequency heart beating and low-frequency morphological changes. I will demonstrate how using our established method of prospective optical gating, in combination with light-sheet microscopy, can allow the synchronised capture of 3D images of the in vivo beating zebrafish heart. However, prospective optical gating alone is limited to snapshots of the heart at chosen target heartbeat phases and only over the scale of tens of minutes.
We have now developed adaptive prospective optical gating technologies that we are using in combination with light-sheet microscopy to enable a range of 3D+time and 3D-timelapse imaging experiments. Our adaptive prospective optical gating technology allows us to carry out 48+ hour, in vivo, 3D-timelapse imaging of the computationally 'frozen' heart across developmental stages, e.g. heart looping, and throughout injury response and repair. Imaging across these timescales is not possible with prospective optical gating alone and phase-locked timelapse imaging is not possible using retrospective optical gating alone: only with our hybrid system are such longitudinal studies possible. Our hybrid prospective-retrospective optical gating system allows researchers to study and understand cardiac development and repair without the use of chemicals or optogenetics to stop or modify the natural heart beating.
We have now developed adaptive prospective optical gating technologies that we are using in combination with light-sheet microscopy to enable a range of 3D+time and 3D-timelapse imaging experiments. Our adaptive prospective optical gating technology allows us to carry out 48+ hour, in vivo, 3D-timelapse imaging of the computationally 'frozen' heart across developmental stages, e.g. heart looping, and throughout injury response and repair. Imaging across these timescales is not possible with prospective optical gating alone and phase-locked timelapse imaging is not possible using retrospective optical gating alone: only with our hybrid system are such longitudinal studies possible. Our hybrid prospective-retrospective optical gating system allows researchers to study and understand cardiac development and repair without the use of chemicals or optogenetics to stop or modify the natural heart beating.
Hope to see many of you there,
Cheers,
Chiara.
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